separation buffer (pbs)  (Corning Life Sciences)

Journal: STAR Protocols

Article Title: Protocol for extracting and isolating porcine bone-marrow-derived macrophages from ribs

doi: 10.1016/j.xpro.2024.103085

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Article Snippet: AutoMACS running buffer (PBS+EDTA) , Miltenyi Biotec , 130-091-221.

Techniques: Recombinant, Saline, Lysis, Isolation, SYBR Green Assay, Software, Sterility, Spectrophotometry, Flow Cytometry

Journal: STAR Protocols

Article Title: Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9

doi: 10.1016/j.xpro.2023.102389

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Article Snippet: Running buffer (autoMACS running buffer; alternatively, use a 2 mM EDTA, 0.5% (wt/vol) BSA/PBS solution) , Miltenyi Biotec , cat. no. 130-091-221.

Techniques: Staining, Virus, Control, Infection, Western Blot, Modification, Purification, Recombinant, Red Blood Cell Lysis, Lysis, Enzyme-linked Immunosorbent Assay, Isolation, Positive Control, Transfection, CRISPR, Sterility, Gene Knock-In, Sequencing, Software, Flow Cytometry, Irradiation

Journal: Cold Spring Harbor protocols

Article Title: Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus

doi: 10.1101/pdb.prot097592

Figure Lengend Snippet: Amphibian Phosphate-Buffered Saline (APBS)

Article Snippet: Open in a separate window Amphibian Phosphate-Buffered Saline (APBS) Reagent Quantity Iscove’s DMEM basal medium, powdered (Gibco 12200–036) 1 package Insulin (Sigma-Aldrich 19278–5mL) 10 mL Nonessential amino acids (Gibco 11140–050) 10 mL Penicillin-streptomycin (Gibco 15070–063) 10 mL Primatone (Sheffield Products Division) 3 mL 2-mercaptoethanol 1 mL NaHCO 3 3.02 g Combine the above ingredients in water.

Techniques: Saline